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278 résultat(s) trouvé(s)
Sous presse, 2016
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Fowler PA - Filis P - Bhattacharya S - Le Bizec B - Antignac JP - Morvan ML - Drake AJ - Sofientini U - O’Shaughnessy PJ
Human anogenital distance: an update on fetal smoke-exposure and integration of the perinatal literature on sex differences
Human Reproduction

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Saraoui T - Fall PA - Leroi F - Antignac JP - Chéreau S - Pilet MF
Inhibition mechanism of Listeria monocytogenes by a bioprotective bacteria Lactococcus piscium CNCM I-4031
Food Microbiology
Listeria monocytogenes is a pathogenic Gram positive bacterium and the etiologic agent of listeriosis, a severe food-borne disease. Lactococcus piscium CNCM I-4031 has the capacity to prevent the growth of L. monocytogenes in contaminated peeled and cooked shrimp. To investigate the inhibititory mechanism, a chemically defined medium (MSMA) based on shrimp composition and reproducing the inhibition observed in shrimp was developed. In co-culture at 26 C, L. monocytogenes was reduced by 3e4 log CFU g-1 after 24 h. We have demonstrated that the inhibition was not due to secretion of extracellular antimicrobial compounds as bacteriocins, organic acids and hydrogen peroxide. Global metabolomic fingerprints of these strains in pure culture were assessed by liquid chromatography coupled with high resolution mass spectrometry. Consumption of glucose, amino-acids, vitamins, nitrogen bases, iron and magnesium was measured and competition for some molecules could be hypothesized. However, after 24 h of co-culture, when inhibition of L. monocytogenes occurred, supplementation of the medium with these compounds did not restore its growth. The inhibition was observed in co-culture but not in diffusion chamber when species were separated by a filter membrane. Taken together, these data indicate that the inhibition mechanism of L. monocytogenes by L. piscium is cell-to-cell contact dependent. © 2015 Elsevier Ltd. All rights reserved.

Sous presse, 2015
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Beucher L - Dervilly-Pinel G - Cesbron N - Penot M - Gicquiau A - Monteau F - Le Bizec B
Specific detection in urine of non-steroidal Selective Androgen Receptor Modulators using supercritical fluid chromatography coupled to ion-mobility mass spectrometry
Drug Testing and Analysis

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Janssens G - Mangelinckx S - Courtheyn D - De Kimpe N - Matthijs B - Le Bizec B
Simultaneous Detection of Androgen and Estrogen Abuse in Breeding Animals by Gas Chromatography−Mass Spectrometry/ Combustion/Isotope Ratio Mass Spectrometry (GC-MS/C/IRMS) Evaluated against Alternative Methods
Journal of Agriculture and Food Chemistry
The administration of synthetic homologues of naturally occurring steroids can be demonstrated by measuring 13C/12C isotopic ratios of their urinary metabolites. Gas chromatography−mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS) was used in this study to appraise in a global approach isotopic deviations of two 17β-testosterone metabolites (17α-testosterone and etiocholanolone) and one 17β-estradiol metabolite (17α-estradiol) together with those of 5-androstene-3β,17α-diol as endogenous reference compound (ERC). Intermediate precisions of 0.35‰, 1.05‰, 0.35‰, and 0.21‰, respectively, were observed (n = 8). To assess the performance of the analytical method, a bull and a heifer were treated with 17β-testosterone propionate and 17β-estradiol-3-benzoate. The sensitivity of the method permitted the demonstration of 17β-estradiol treatment up to 24 days. For 17β-testosterone treatment, the detection windows were 3 days and 24 days for the bull and the heifer, respectively. The capability of GC-MS/C/IRMS to demonstrate natural steroid abuse for urinary steroids was eventually compared to those of mass spectrometry (LC-MS/MS) when measuring intact steroid esters in blood and hair. © 2015 American Chemical Society.

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Cariou R - Veyrand V - Yamada A - Berrebi A - Zalko D - Durand S - Pollono A - Marchand M - Leblanc JC - Antignac JP - Le Bizec B
Perfluoroalkyl acid (PFAA) levels and profiles in breast milk, maternal and cord serum of French women and their newborns
Environment International
One major concern regarding perfluoroalkyl acids (PFAAs) is their potential role in onset of health troubles consecutive to early exposure during the perinatal period. In the present work, the internal exposure levels of 18 targeted PFAAs were determined in ca. 100 mother-newborn pairs recruited in France between 2010 and 2013. In serum, the cumulated concentrations of the 7 most frequently detected compounds were 5.70 ng/mL and 2.83 ng/mL (median values) in maternal and cord serum, respectively. Perfluorooctanesulfonic acid (PFOS), perfluorooctanoic acid (PFOA), perfluorohexylesulfonic acid (PFHxS) and perfluorononanoic acid (PFNA) contributed to around 90% of the total PFAAs contamination, with concentration levels and contamination profiles in accordance with other published work in Europe. Levels measured in breast milk were far lower (20 to 150 fold) than those determined in serum. Associations between the different monitored substances as well as between levels determined in the different investigated biological matrices mostly do not appear statistically significant. The estimated materno-foetal transfer would be thus substance-dependant, mainly driven by the physico-chemical properties of the different PFAAs (nature of polar group and length of alkylated side chain). We conclude that trans-placental passage and breastfeeding are both significant routes of human exposure to PFAAs. © 2015 Published by Elsevier Ltd.

Sous presse, 2015
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Maurice N - Olry JC - Cariou R - Dervilly-Pinel G - Le Bizec B - Travel A - Jondreville C - Schroeder H
Short-term effects of a perinatal exposure to the HBCDD a-isomer in rats: assessment of early motor and sensorial development and level of anxiety in pups
Neurotoxicology and Teratology
The present study investigated the developmental neurotoxicity of an early exposure to α-HBCDD through the ingestion of contaminated hen's egg in pregnant and lactating Wistar female rats. Hens were given α-HBCDD contaminated feed (40 ng/g fresh matter) for 5 and 10 days, which produced eggs with HBCDD content of 33 and 102 ng/g lipid weight, respectively. Female rats were administered daily p.o. with an appropriate volume of the whole egg from the day of fertilization (GD0) to the weaning day for pups (PND21). Fetuses and pups were thus exposed continuously to α-HBCDD via the dam over a whole 42-day period that included both gestation and lactation. The administered egg volume was calculated on the basis of daily egg consumption in humans (0.7 egg/person/day) and duration of gestation and lactation in both species, which led animals to be exposed to α-HBCDD at levels of 22 and 66 ng/kg/day, respectively. Neurobehavioral development of pups was investigated from PND3 to PND25 using various tasks including the righting reflex (PND4), the grasping reflex (PND5), the negative geotaxis (PND9), the forelimb grip strength test (PND10) and the locomotor coordination test (PND20). Pup ultrasonic vocalizations were also recorded daily from PND4 to PND14. After weaning, behaviors related to spontaneous locomotor activity and anxiety were examined in the open-field (PND25) and in an elevated-plus maze (PND26), respectively. The results showed a significant decrease in bodyweight of pups exposed to the lower HBCDD level from PND3 to PND28, whereas the weight of rat pups given 66 ng/kg/day of HBCDD was not different from controls. During the first 3 weeks of life, impairments in motor maturation of pups were observed in a dose-dependent manner depending on the test, whereas no significant differences were reported between male and female pups. At PND26, the anxiety level of female rats exposed to the lowest dose ofHBCDD (22 ng/kg/day) was significantly reduced whereas it remained unchanged in males.No significant variations were measured in rats exposed to the higher level of HBCDD (66 ng/kg/day). These results suggest the potent developmental neurotoxicity of an early chronic exposure to the HBCDD α-isomer through the ingestion of hen's eggs contaminated with this pollutant and question the long-lasting consequences of this exposure on behavior abilities and brain functioning in adulthood. © 2015 Elsevier Inc. All rights reserved.

Sous presse, 2015
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Le Boucher C - Gagnaire V - Briard-Bion V - Jardin J - Maillard MB - Dervilly-Pinel G - Le Bizec B - Lortal S - Jeanson S - Thierry A
The Spatial Distribution of Lactococcus lactis Colonies Modulates the Ripening of a Model Cheese
Applied and Environmental Microbiology

Sous presse, 2015
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Svechnikov K - Savchuk I - Morvan ML - Antignac JP - Le Bizec B - Söder O
Phthalates exert multiple effects on Leydig cell steroidogenesis
Hormone Research in Pediatrics
Humans are significantly exposed to phthalates via food packaging, cosmetics and medical devices such as tubings and catheters. Testicular Leydig cells (LCs) are suggested to be among the main targets of phthalate toxicity in the body. However, their sensitivity to phthalates is species-dependent. This paper describes the response of the LCs from different species (mouse, rat and human) to phthalate exposure in different experimental paradigms (in vivo, ex vivo and in vitro), with particular focus on mechanisms of phthalate action on LC steroidogenesis. A comprehensive analysis of the impact of phthalate diesters and phthalate monoesters on LCs in different stages of their development is presented and possible mechanisms of phthalates action are discussed. Finally novel, not yet fully elucidated sites of action of phthalate monoesters on the backdoor pathway of 5α-dihydrotestosterone biosynthesis in immature mouse LCs and their effects on steroidogenesis and redox state in adult mouse LCs are reported. © 2015 S. Karger AG, Basel.

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Kouassi Nzoughet J - Dervilly-Pinel G - Gallart-Ayala H - Biancotto G - Le Bizec B
Hydrophilic Interaction (HILIC) and Reverse Phase Liquid Chromatography (RPLC) - High Resolution MS for characterizing lipids profile disruption in serum of anabolic implanted bovines
Glycerophospholipids have been highlighted as the major lipids class affected by trenbolone acetate/estradiol implant administration in bovines. Non-targeted hydrophilic interaction chromatography (HILIC) and reverse phase liquid chromatography (RPLC) coupled to high resolution mass spectrometry have been successfully applied for characterizing lipid profile disruption in serum of implanted bovines. HILIC data pointed towards significant decrease of C22 fatty acids in implanted animals, i.e., C22:3 (p0.05), C22:4 (p0.05) and C22:6 (p0.01), whilst RPLC data confirmed depletion of glycerophospholipids (phosphatidylglycerols, phosphatidylcholine, phosphatidic acid and phosphatidylethanolamine) with C22 fatty acid chains. Using these two complementary methods, complex lipids with the same alkyl chain have been putatively characterized and could further serve to understand the biological mechanism of illicit administration of trenbolone acetate/estradiol implant. These metabolites underpin glycerophospholipid metabolism as potential pathway, which was identified using metabolomic pathway analysis and MetExplore platforms. We hypothesized that implantation of exogenous androgenic and estrogenic steroids induces a depletion of glycerophospholipids with C22 FA chains, by decreasing high-density lipoproteins, the main transporters of glycerophospholipids in plasma. © Springer Science+Business Media New York 2015.

Avril 2015
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Dervilly-Pinel G - Prevost S - Sérée L - Le Bizec B
Vers des stratégies analytiques globales et non ciblées de recherche de résidus de substances interdites en élevage
ANSES Bulletin Epidémiologique – Santé animale et alimentation n°68/Spécial Vigilance

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